General Information:

Id: 785
Diseases: Parkinson disease
Homo sapiens
BTO:0000793 SH-SY5Y cell; BTO:0002104 BE(2)M17 cell
article
Reference: Lim MK et al.(2007) Parkin interacts with LIM Kinase 1 and reduces its cofilin-phosphorylation activity via ubiquitination Exp. Cell Res. 313: 2858-2874 [PMID: 17512523]

Interaction Information:

Comment Cofilin binds to actin filaments and promotes actin filament turnover.
Formal Description
Interaction-ID: 4391

gene/protein

CFL1

increases_activity of

Drugbank entries Show/Hide entries for CFL1
Comment Cofilin binds to actin filaments and promotes actin filament turnover.
Formal Description
Interaction-ID: 4392

gene/protein

CFL1

interacts (colocalizes) with

cellular component

actin filament

Drugbank entries Show/Hide entries for CFL1
Comment LIMK1 inactivate cofilin through phosphorylation and inhibit its actin depolymerizing activity.
Formal Description
Interaction-ID: 4394

gene/protein

LIMK1

decreases_activity of

gene/protein

CFL1

Drugbank entries Show/Hide entries for CFL1
Comment LIMK1 inactivate cofilin through phosphorylation and inhibit its actin depolymerizing activity.
Formal Description
Interaction-ID: 4395

gene/protein

LIMK1

increases_phosphorylation of

gene/protein

CFL1

Drugbank entries Show/Hide entries for CFL1
Comment LIMK1 inactivate cofilin through phosphorylation and inhibit its actin depolymerizing activity.
Formal Description
Interaction-ID: 4396

gene/protein

LIMK1

decreases_activity of

through inhibition of cofilin
Comment Parkin interacts with LIMK1 also in the presence of CHIP. Surprisingly, the presence of increasing CHIP potentiated LIMK1 association with parkin in a dose-dependent manner.
Formal Description
Interaction-ID: 4397

gene/protein

PARK2

interacts (colocalizes) with

gene/protein

LIMK1

also in the presence of CHIP
Comment Parkin interacts with LIMK1 and LIMK2.
Formal Description
Interaction-ID: 4398

gene/protein

PARK2

interacts (colocalizes) with

gene/protein

LIMK2

Comment Parkin interacts with LIMK1 also in the presence of CHIP. Surprisingly, the presence of increasing CHIP potentiated LIMK1 association with parkin in a dose-dependent manner.
Formal Description
Interaction-ID: 4412

gene/protein

STUB1

increases_quantity of

complex/PPI

PARK2-LIMK1 complex

Comment Parkin also interacts with the F-box/WD repeats domain protein, hSel-10. Sel10 did not alter the interaction between parkin and LIMK1.
Formal Description
Interaction-ID: 4414

gene/protein

PARK2

interacts (colocalizes) with

gene/protein

FBXW7

Comment Immunoprecipitation of FLAG-LIMK1 resulted in CO-IP of HAT-CHIP but not of HAT-hSel10.
Formal Description
Interaction-ID: 4415

gene/protein

LIMK1

interacts (colocalizes) with

gene/protein

STUB1

Comment Immunoprecipitation of FLAG-LIMK1 resulted in CO-IP of HAT-CHIP but not of HAT-hSel10.
Formal Description
Interaction-ID: 4416

gene/protein

LIMK1

NOT interacts (colocalizes) with

gene/protein

FBXW7

Comment Immunoprecipitation of FLAG-Hsp70 resulted in Co-IP of HA-parkin and HA-LIMK1.
Formal Description
Interaction-ID: 4417

gene/protein

HSP70

interacts (colocalizes) with

gene/protein

PARK2

Comment Immunoprecipitation of FLAG-Hsp70 resulted in Co-IP of HA-parkin and HA-LIMK1.
Formal Description
Interaction-ID: 4418

gene/protein

HSP70

interacts (colocalizes) with

gene/protein

LIMK1

Comment Parkin enhanced ubiquitination of LIMK1 in vitro and only in neuroblastoma-derived BE(2)-M17 cells, but not in embryonic kidney-derived HEK293 cells. Instead, parkin reduced LIMK1-ubiquitination in HEK293 cells.
Formal Description
Interaction-ID: 4426

gene/protein

PARK2

increases_ubiquitination/sumoylation of

gene/protein

LIMK1

in vitro or in BE(2)-M17 cells, but not in HEK293 cells
Comment Parkin modulates the level of phosphocofilin by negatively regulating LIMK1 activity in a cell type-dependent manner.
Formal Description
Interaction-ID: 4431

gene/protein

PARK2

decreases_activity of

gene/protein

LIMK1

in a cell type-dependent manner
Comment Parkin reduces LIMK1-induced actin filament aggregation through interaction and ubiquitination of LIMK1.
Formal Description
Interaction-ID: 4443

gene/protein

PARK2

decreases_activity of

through ubiquitination of LIMK1
Comment Parkin reduces LIMK1-induced actin filament aggregation through interaction and ubiquitination of LIMK1.
Formal Description
Interaction-ID: 4446

gene/protein

PARK2

increases_activity of

through ubiquitination of LIMK1
Comment Parkin reduces LIMK1-induced actin filament aggregation through interaction and ubiquitination of LIMK1.
Formal Description
Interaction-ID: 4449

gene/protein

LIMK1

increases_activity of

Comment LIMK1 can phosphorylate itself.
Formal Description
Interaction-ID: 4452

gene/protein

LIMK1

increases_phosphorylation of

gene/protein

LIMK1

Comment LIMK1 did not phosphorylate parkin, whereas parkin was strongly phosphorylated by PKA.
Formal Description
Interaction-ID: 4455

gene/protein

LIMK1

NOT increases_phosphorylation of

gene/protein

PARK2

Comment LIMK1 did not phosphorylate parkin, whereas parkin was strongly phosphorylated by PKA.
Formal Description
Interaction-ID: 4456

complex/PPI

Protein kinase A

increases_phosphorylation of

gene/protein

PARK2

Comment Parkin is auto-ubiquitinated in vitro and in cell lines.
Formal Description
Interaction-ID: 4457

gene/protein

PARK2

increases_ubiquitination/sumoylation of

gene/protein

PARK2

Comment LIMK1 could inhibit the ubiquitin E3 ligase activity of parkin in vitro and in HEK293 cells.
Formal Description
Interaction-ID: 4459

gene/protein

LIMK1

decreases_activity of

gene/protein

PARK2

Comment LIMK1 could inhibit the ubiquitin E3 ligase activity of parkin in vitro and in HEK293 cells.
Formal Description
Interaction-ID: 4462

gene/protein

LIMK1

decreases_ubiquitination/sumoylation of

gene/protein

PARK2

Comment LIMK1 inactivate cofilin through phosphorylation and inhibit its actin depolymerizing activity.
Formal Description
Interaction-ID: 9356

gene/protein

LIMK1

increases_activity of

through inhibition of cofilin