Dcdc2 protein localizes to the primary cilium in primary rat hippocampal neurons and it can be found within close proximity to the ciliary kinesin-2 subunit Kif3a.
Overexpression of DCDC2 increases ciliary length and activates Shh signaling, whereas downregulation of Dcdc2 expression enhances Wnt signaling, consistent with a functional role in ciliary signaling.
Overexpression of DCDC2 increases ciliary length and activates Shh signaling, whereas downregulation of Dcdc2 expression enhances Wnt signaling, consistent with a functional role in ciliary signaling.
Overexpression of DCDC2 increases ciliary length and activates Shh signaling, whereas downregulation of Dcdc2 expression enhances Wnt signaling, consistent with a functional role in ciliary signaling.
Lysates from rat primary hippocampal neurons were pulled down with an antibody against DCDC2. Probing with Kif3a antibody revealed that Kif3a could be precipitated together with Dcdc2.
Key microarray results were further confirmed by quantitative real-time PCR (qRT-PCR). For the verification, five genes were selected: Pdgfra which was the most upregulated gene, Cdc2 which is involved in the same pathway as Pdgfra, two kinesin family genes Kif4 and Kif2c, and Hhip that is involved in Sonic Hedgehog signaling. Changes in gene expression measured by qRT-PCR were fully consistent with the data obtained by microarray analysis.
Key microarray results were further confirmed by quantitative real-time PCR (qRT-PCR). For the verification, five genes were selected: Pdgfra which was the most upregulated gene, Cdc2 which is involved in the same pathway as Pdgfra, two kinesin family genes Kif4 and Kif2c, and Hhip that is involved in Sonic Hedgehog signaling. Changes in gene expression measured by qRT-PCR were fully consistent with the data obtained by microarray analysis.
Key microarray results were further confirmed by quantitative real-time PCR (qRT-PCR). For the verification, five genes were selected: Pdgfra which was the most upregulated gene, Cdc2 which is involved in the same pathway as Pdgfra, two kinesin family genes Kif4 and Kif2c, and Hhip that is involved in Sonic Hedgehog signaling. Changes in gene expression measured by qRT-PCR were fully consistent with the data obtained by microarray analysis.
Key microarray results were further confirmed by quantitative real-time PCR (qRT-PCR). For the verification, five genes were selected: Pdgfra which was the most upregulated gene, Cdc2 which is involved in the same pathway as Pdgfra, two kinesin family genes Kif4 and Kif2c, and Hhip that is involved in Sonic Hedgehog signaling. Changes in gene expression measured by qRT-PCR were fully consistent with the data obtained by microarray analysis.
Key microarray results were further confirmed by quantitative real-time PCR (qRT-PCR). For the verification, five genes were selected: Pdgfra which was the most upregulated gene, Cdc2 which is involved in the same pathway as Pdgfra, two kinesin family genes Kif4 and Kif2c, and Hhip that is involved in Sonic Hedgehog signaling. Changes in gene expression measured by qRT-PCR were fully consistent with the data obtained by microarray analysis.
CIDeR