General Information:

Id: 58
Diseases: Diabetes mellitus, type I - [OMIM]
Rattus norvegicus
BTO:0001185 RINm5F cell
article
Reference: Song MY et al.(2009) Sulforaphane protects against cytokine- and streptozotocin-induced beta-cell damage by suppressing the NF-kappaB pathway. Toxicol. Appl. Pharmacol. 235: 57-67 [PMID: 19071154]

Interaction Information:

Comment Sulforaphane (SFN) increased Nrf2 protein levels in the nuclear fraction, whereas Nrf2 was located predominantly in the cytoplasm of control cells.
Formal Description
Interaction-ID: 164

drug/chemical compound

Sulforaphane

increases_transport of

gene/protein

NFE2L2

into nucleus
Comment Sulforaphane (SFN) greatly increased expression of the Nrf2 target genes HO-1, GSTA4, GSTM1, NQO1, GCLC, and GCLM.
Formal Description
Interaction-ID: 168

drug/chemical compound

Sulforaphane

increases_expression of

gene/protein

HMOX1

Drugbank entries Show/Hide entries for HMOX1
Comment Sulforaphane (SFN) greatly increased expression of the Nrf2 target genes HO-1, GSTA4, GSTM1, NQO1, GCLC, and GCLM.
Formal Description
Interaction-ID: 169

drug/chemical compound

Sulforaphane

increases_expression of

gene/protein

GSTA4

Drugbank entries Show/Hide entries for GSTA4
Comment Sulforaphane (SFN) greatly increased expression of the Nrf2 target genes HO-1, GSTA4, GSTM1, NQO1, GCLC, and GCLM.
Formal Description
Interaction-ID: 170

drug/chemical compound

Sulforaphane

increases_expression of

gene/protein

NQO1

Drugbank entries Show/Hide entries for NQO1
Comment Sulforaphane (SFN) greatly increased expression of the Nrf2 target genes HO-1, GSTA4, GSTM1, NQO1, GCLC, and GCLM.
Formal Description
Interaction-ID: 171

drug/chemical compound

Sulforaphane

increases_expression of

gene/protein

GCLC

Drugbank entries Show/Hide entries for GCLC
Comment Sulforaphane (SFN) greatly increased expression of the Nrf2 target genes HO-1, GSTA4, GSTM1, NQO1, GCLC, and GCLM.
Formal Description
Interaction-ID: 172

drug/chemical compound

Sulforaphane

increases_expression of

gene/protein

GCLM

Drugbank entries Show/Hide entries for GCLM
Comment iNOS and COX-2 mRNA and protein expression were markedly increased in cells treated with cytokines (IL-1-beta and IFN-gamma), and suppressed in cells pretreated with SFN.
Formal Description
Interaction-ID: 173

gene/protein

IL1B

increases_expression of

gene/protein

NOS2

Drugbank entries Show/Hide entries for IL1B or NOS2
Comment iNOS and COX-2 mRNA and protein expression were markedly increased in cells treated with cytokines (IL-1-beta and IFN-gamma), and suppressed in cells pretreated with SFN.
Formal Description
Interaction-ID: 174

gene/protein

IL1B

increases_expression of

gene/protein

PTGS2

Drugbank entries Show/Hide entries for IL1B or PTGS2
Comment iNOS and COX-2 mRNA and protein expression were markedly increased in cells treated with cytokines (IL-1-beta and IFN-gamma), and suppressed in cells pretreated with SFN.
Formal Description
Interaction-ID: 175

drug/chemical compound

Sulforaphane

decreases_expression of

gene/protein

PTGS2

in cytokine treated cells
Drugbank entries Show/Hide entries for PTGS2
Comment iNOS and COX-2 mRNA and protein expression were markedly increased in cells treated with cytokines (IL-1-beta and IFN-gamma), and suppressed in cells pretreated with SFN.
Formal Description
Interaction-ID: 176

drug/chemical compound

Sulforaphane

decreases_expression of

gene/protein

NOS2

in cytokine treated cells
Drugbank entries Show/Hide entries for NOS2
Comment Cytokine-treated cells showed increased levels of p65 and p50 subunits in their nuclei, compared with unstimulated cells.
Formal Description
Interaction-ID: 178

gene/protein

IL1B

increases_transport of

gene/protein

RELA

into nucleus
Drugbank entries Show/Hide entries for IL1B or RELA
Comment Cytokine-treated cells showed increased levels of p65 and p50 subunits in their nuclei, compared with unstimulated cells.
Formal Description
Interaction-ID: 179

gene/protein

IL1B

increases_transport of

gene/protein

NFKB1

into nucleus
Drugbank entries Show/Hide entries for IL1B or NFKB1
Comment Cytokine-induced activation of NF-kappa-B was markedly suppressed by pretreatment with SFN.
Formal Description
Interaction-ID: 180

drug/chemical compound

Sulforaphane

decreases transport of

gene/protein

RELA

into nucleus; in cytokine treated cells
Drugbank entries Show/Hide entries for RELA
Comment Cytokine-induced activation of NF-kappa-B was markedly suppressed by pretreatment with SFN.
Formal Description
Interaction-ID: 181

drug/chemical compound

Sulforaphane

decreases transport of

gene/protein

NFKB1

into nucleus; in cytokine treated cells
Drugbank entries Show/Hide entries for NFKB1
Comment Sulforaphane (SFN) caused Nrf2 activation.
Formal Description
Interaction-ID: 182

drug/chemical compound

Sulforaphane

increases_activity of

gene/protein

NFE2L2

Comment Overexpression of Nrf2 caused dramatic decreases in the induction of iNOS and COX-2 mRNA expression by cytokines.
Formal Description
Interaction-ID: 183

gene/protein

NFE2L2

decreases_expression of

gene/protein

NOS2

if Nrf2 is overexpressed in cytokine treated cells
Drugbank entries Show/Hide entries for NOS2
Comment Overexpression of Nrf2 caused dramatic decreases in the induction of iNOS and COX-2 mRNA expression by cytokines.
Formal Description
Interaction-ID: 184

gene/protein

NFE2L2

decreases_expression of

gene/protein

PTGS2

if Nrf2 is overexpressed in cytokine treated cells
Drugbank entries Show/Hide entries for PTGS2
Comment Cytokine (Il-1-beta and IFN-gamma) treatment induced oxidative stress analysed by H(2)O(2) formation.
Formal Description
Interaction-ID: 185

gene/protein

IL1B

increases_activity of

Drugbank entries Show/Hide entries for IL1B
Comment Cytokine (Il-1-beta and IFN-gamma) treatment induced oxidative stress analysed by H(2)O(2) formation.
Formal Description
Interaction-ID: 187

gene/protein

IL1B

increases_quantity of

drug/chemical compound

H2O2

Drugbank entries Show/Hide entries for IL1B
Comment H(2)O(2) production was abolished when RIN cells were either pretreated with SFN or transfected with Nrf2 before incubation with cytokines.
Formal Description
Interaction-ID: 189

drug/chemical compound

Sulforaphane

decreases_quantity of

drug/chemical compound

H2O2

in cytokine treated cells
Comment H(2)O(2) production was abolished when RIN cells were either pretreated with SFN or transfected with Nrf2 before incubation with cytokines.
Formal Description
Interaction-ID: 190

gene/protein

NFE2L2

decreases_quantity of

drug/chemical compound

H2O2

if Nrf2 is overexpressed in cytokine treated cells
Comment Cytokine (IL-1-beta and IFN-gamma) treatment showed increased DNA binding and transcriptional activities of NF-kappa-B.
Formal Description
Interaction-ID: 269

gene/protein

IL1B

increases_activity of

Drugbank entries Show/Hide entries for IL1B