General Information:

Id: 551
Diseases: Breast cancer - [OMIM]
Diabetes mellitus, type II - [OMIM]
Insulin resistance
Obesity - [OMIM]
Homo sapiens
female
primary human breast adipose stromal cells
article
Reference: Brown KA et al.(2009) Subcellular localization of cyclic AMP-responsive element binding protein-regulated transcription coactivator 2 provides a link between obesity and breast cancer in postmenopausal women Cancer Res. 69: 5392-5399 [PMID: 19509226]

Interaction Information:

Comment Treatment of human adipose stromal cells with forskolin and phorbol 12-myristate 13-acetate (PMA), to mimic prostaglandin E(2), resulted in a 5.5-fold increase in aromatase mRNA.
Formal Description
Interaction-ID: 2843

drug/chemical compound

Prostaglandin E2

increases_expression of

gene/protein

CYP19A1

in adipose stromal cells
Drugbank entries Show/Hide entries for CYP19A1
Comment Aromatase promoter II (PII) activity assays showed that CRTC2 in addition to forskolin/PMA treatment significantly increased PII-induced activity.
Formal Description
Interaction-ID: 2866

gene/protein

CRTC2

increases_activity of

gene/protein

CYP19A1

in adipose stromal cells
Drugbank entries Show/Hide entries for CYP19A1
Comment CRTC2 activity is largely mediated by its subcellular localization, under resting conditions CRCT2 is mainly located in the cytoplasm.
Formal Description
Interaction-ID: 2867

gene/protein

CRTC2

is localized in

cellular component

cytoplasm

in adipose stromal cells; under resting conditions
Comment Treatment of human adipose stromal cells with forskolin and phorbol 12-myristate 13-acetate (PMA), to mimic prostaglandin E2, resulted in nuclear translocation of CRTC2.
Formal Description
Interaction-ID: 2868

drug/chemical compound

Prostaglandin E2

increases_transport of

gene/protein

CRTC2

in adipose stromal cells; into the nucleus
Comment Stimulation of AMPK activity in forskolin/PMA treated cells with AICAR (an AMP analogue) resulted in a significant decrease in aromatase expression.
Formal Description
Interaction-ID: 2870

complex/PPI

AMPK

decreases_expression of

gene/protein

CYP19A1

in adipose stromal cells
Drugbank entries Show/Hide entries for CYP19A1
Comment The forskolin/PMA-mediated translocation of CRTC2 to the nucleus was prevented by treatment with AICAR.
Formal Description
Interaction-ID: 2871

drug/chemical compound

AICAR

affects transport of

gene/protein

CRTC2

in adipose stromal cells; into the nucleus
Drugbank entries Show/Hide entries for AICAR
Comment Stimulation of the protein kinase A and C pathways in human adipose stromal cells results in nuclear translocation of CRTC2 and this is accompanied by an increase in CRTC2-dependent activation of aromatase PII.
Formal Description
Interaction-ID: 2872

increases_transport of

gene/protein

CRTC2

in adipose stromal cells; into the nucleus
Comment The forskolin/PMA-mediated translocation of CRTC2 to the nucleus was prevented by cotransfection with LKB1 and knockdown of LKB1 using siRNA was sufficient to cause nuclear localization of CRTC2.
Formal Description
Interaction-ID: 2873

gene/protein

STK11

affects transport of

gene/protein

CRTC2

in adipose stromal cells; into the nucleus
Comment In human adipose stromal cells forskolin/PMA treatment ( to mimic prostaglandin E2) resulted in a significant decrease in LKB1 transcript and protein expression and this was associated with a significant decrease in phosphorylation of AMPK at Thr172.
Formal Description
Interaction-ID: 2875

drug/chemical compound

Prostaglandin E2

affects_expression of

gene/protein

STK11

in adipose stromal cells
Comment Total AMPK protein expression was significantly decreased in response to forskolin/PMA.
Formal Description
Interaction-ID: 2879

drug/chemical compound

Prostaglandin E2

decreases_quantity of

complex/PPI

AMPK

in adipose stromal cells
Comment Phosphorylation of the AMPK alpha subunits at Ser485, which is inhibitory of AMPK activity, significantly increased with forskolin/PMA treatment.
Formal Description
Interaction-ID: 2880

drug/chemical compound

Prostaglandin E2

decreases_activity of

complex/PPI

AMPK

in adipose stromal cells; via phosphorylation of the AMPK alpha subunits at Ser485
Comment Inhibition of the LKB1/AMPK pathway by factors that stimulate the protein kinase A and C pathways contributes to the CRTC2-dependent PII-driven stimulation of aromatase expression in human adipose stromal cells.
Formal Description
Interaction-ID: 2882

decreases_activity of

complex/PPI

AMPK

in adipose stromal cells
Comment Leptin induces aromatase expression via CRTC2 translocation to the nucleus and inhibition of LKB1 expression in human adipose stromal cells.
Formal Description
Interaction-ID: 2883

gene/protein

LEP

increases_expression of

gene/protein

CYP19A1

in adipose stromal cells
Drugbank entries Show/Hide entries for CYP19A1
Comment Leptin induces aromatase expression via CRTC2 translocation to the nucleus and inhibition of LKB1 expression in human adipose stromal cells.
Formal Description
Interaction-ID: 2884

gene/protein

LEP

decreases_expression of

gene/protein

STK11

in adipose stromal cells
Comment Leptin induces aromatase expression via CRTC2 translocation to the nucleus and inhibition of LKB1 expression in human adipose stromal cells.
Formal Description
Interaction-ID: 2888

gene/protein

LEP

increases_transport of

gene/protein

CRTC2

in adipose stromal cells; into the nucleus
Comment Adiponectin down-regulates stimulated aromatase expression in human adipose stromal cells via CRTC2.
Formal Description
Interaction-ID: 2891

gene/protein

ADIPOQ

decreases_expression of

gene/protein

CYP19A1

in adipose stromal cells; via CRTC2
Drugbank entries Show/Hide entries for CYP19A1
Comment Adiponectin significantly increased LKB1 expression and activity.
Formal Description
Interaction-ID: 2896

gene/protein

ADIPOQ

increases_expression of

gene/protein

STK11

in adipose stromal cells
Comment Stimulation of the protein kinase A and C pathways in human adipose stromal cells results in nuclear translocation of CRTC2 and this is accompanied by an increase in CRTC2-dependent activation of aromatase PII.
Formal Description
Interaction-ID: 12832

increases_transport of

gene/protein

CRTC2

into the nucleus
Comment Inhibition of the LKB1/AMPK pathway by factors that stimulate the protein kinase A and C pathways contributes to the CRTC2-dependent PII-driven stimulation of aromatase expression in human adipose stromal cells.
Formal Description
Interaction-ID: 12833

decreases_activity of

gene/protein

STK11

in adipose stromal cells
Comment Inhibition of the LKB1/AMPK pathway by factors that stimulate the protein kinase A and C pathways contributes to the CRTC2-dependent PII-driven stimulation of aromatase expression in human adipose stromal cells.
Formal Description
Interaction-ID: 12834

decreases_activity of

gene/protein

STK11

in adipose stromal cells
Comment Inhibition of the LKB1/AMPK pathway by factors that stimulate the protein kinase A and C pathways contributes to the CRTC2-dependent PII-driven stimulation of aromatase expression in human adipose stromal cells.
Formal Description
Interaction-ID: 12835

decreases_activity of

complex/PPI

AMPK

in adipose stromal cells