General Information:

Id: 543 (click here to show other Interactions for entry)
Diseases: Parkinson disease
Homo sapiens
BTO:0000567 HeLa cell; BTO:0000793 SH-SY5Y cell
article
Reference: Geisler S et al.(2010) PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1 Nat. Cell Biol. 12: 119-131 [PMID: 20098416]

Interaction Information:

Comment Treatment with the uncoupler CCCP induced complete translocation of transfected Parkin from the cytoplasm to depolarized mitochondria. Parkin localized to mitochondria induces mitophagy.
Formal Description
Interaction-ID: 2744

drug/chemical compound

CCCP

increases_transport of

gene/protein

PARK2

into mitochondria
Comment Treatment with the uncoupler CCCP induced complete translocation of transfected Parkin from the cytoplasm to depolarized mitochondria. Parkin localized to mitochondria induces mitophagy.
Formal Description
Interaction-ID: 2785

gene/protein

PARK2

increases_activity of

if Parkin is localized in mitochondria
Comment PINK1 knockdown significantly abolished Parkin recruitment to mitochondria after 2h of CCCP treatment as well as mitophagic clearance within 24 h.
Formal Description
Interaction-ID: 2824

gene/protein

PINK1

affects transport of

gene/protein

PARK2

into mitochondria; after CCCP treatment
Comment VDAC1 is ubiquitylated in a Parkin-dependent manner after CCCP treatment. None of the analysed pathogenic Parkin mutants were able to modify VDAC1.
Formal Description
Interaction-ID: 2850

gene/protein

PARK2

increases_ubiquitination/sumoylation of

gene/protein

VDAC1

after CCCP treatment
Drugbank entries Show/Hide entries for VDAC1
Comment Partial silencing of VDAC1 resulted in significantly reduced translocation of Parkin from the cytosol to damaged mitochondria after 2 h of CCCP treatment. After 24 h of CCCP treatment, partial knockdown of VDAC1 also significantly prevented mitochondrial clearance.
Formal Description
Interaction-ID: 2857

gene/protein

VDAC1

affects transport of

gene/protein

PARK2

into mitochondria; after CCCP treatment
Drugbank entries Show/Hide entries for VDAC1
Comment Silencing of TRAP1 had no effect on Parkin translocation or on mitochondrial clearance.
Formal Description
Interaction-ID: 2859

gene/protein

TRAP1

NOT affects transport of

gene/protein

PARK2

into mitochondria; after CCCP treatment