General Information:

Id: 539
Diseases: Diabetes mellitus, type II - [OMIM]
Insulin resistance
Mus musculus
GPAT1-/- mouse
article
Reference: Lewin TM et al.(2008) Mice deficient in mitochondrial glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition Biochim. Biophys. Acta 1781: 352-358 [PMID: 18522808]

Interaction Information:

Comment GPAT activity from wild-type and GPAT1-/- mice is identical in heart microsomes. Hearts from GPAT1-/- mice lack GPAT1 mRNA, but retain NEM-resistant GPAT activity in mitochondria. Absence of GPAT1 diminishes myocardial triacylglycerol (TAG) accumulation in response to a high-sucrose diet.
Formal Description
Interaction-ID: 2753

gene/protein

GPAM

affects_activity of

in cardiac muscle; after high-sucrose diet
Comment Absence of GPAT1 diminishes myocardial TAG accumulation in response to a high-fat diet.
Formal Description
Interaction-ID: 2754

gene/protein

GPAM

affects_activity of

in cardiac muscle; after high-fat diet
Comment Although weeks of high-sucrose feeding did not change GPAT1 mRNA expression or NEM-resistant activity in mitochondria, myocardial TAG content increased 2 -fold in male and female wild-type mice.
Formal Description
Interaction-ID: 2755

environment

high-sucrose diet

NOT affects_expression of

gene/protein

GPAM

in cardiac muscle; in wild-type mice
Comment Although weeks of high-sucrose feeding did not change GPAT1 mRNA expression or NEM-resistant activity in mitochondria, myocardial TAG content increased 2 -fold in male and female wild-type mice.
Formal Description
Interaction-ID: 2756

environment

high-sucrose diet

NOT affects_activity of

gene/protein

GPAM

in cardiac muscle; in wild-type mice
Comment Although weeks of high-sucrose feeding did not change GPAT1 mRNA expression or NEM-resistant activity in mitochondria, myocardial TAG content increased 2 -fold in male and female wild-type mice.
Formal Description
Interaction-ID: 2757

environment

high-sucrose diet

increases_quantity of

drug/chemical compound

Triacylglycerol

in cardiac muscle; in wild-type mice: in male mice; in female mice
Comment In sucrose-fed male GPAT1-/- mice, heart TAG content did not increase as compared to mice fed the control diet.
Formal Description
Interaction-ID: 2759

environment

high-sucrose diet

NOT increases_quantity of

drug/chemical compound

Triacylglycerol

in cardiac muscle; in male GPAT1-/- mice
Comment In sucrose-fed female GPAT1-/- mice, myocardial TAG only increased 45 %, less than in wild-type mice.
Formal Description
Interaction-ID: 2760

environment

high-sucrose diet

increases_quantity of

drug/chemical compound

Triacylglycerol

in cardiac muscle; in female GPAT1-/- mice
Comment In sucrose-fed male GPAT1-/- mice, plasma TAG and cholesterol were significantly lower than in wild-type mice.
Formal Description
Interaction-ID: 2761

gene/protein

GPAM

affects_quantity of

drug/chemical compound

Triacylglycerol

in blood; in male GPAT1-/- mice after high-sucrose diet
Comment In sucrose-fed female GPAT1-/- mice, values for plasma free fatty acid, TAG, and cholesterol did not differ significantly from control or from treated wild-type mice.
Formal Description
Interaction-ID: 2772

gene/protein

GPAM

NOT affects_quantity of

drug/chemical compound

Fatty acid

in blood; in female GPAT1-/- mice after high-sucrose diet
Comment Consistent with the diminished TAG synthesis observed in the absence of GPAT1, sucrose-fed female GPAT1-/- mice had 40% lower content of liver TAG compared to wild-type mice.
Formal Description
Interaction-ID: 2774

gene/protein

GPAM

affects_quantity of

drug/chemical compound

Triacylglycerol

in liver; in female GPAT1-/- mice after high-sucrose diet
Comment Although plasma TAG and cholesterol concentrations were lower in male GPAT1-/- mice fed the sucrose diet and unchanged in female GPAT1-/- mice, VLDL secretion rates were 30% lower in both sexes compared to wild-type mice.
Formal Description
Interaction-ID: 2775

gene/protein

GPAM

affects_activity of

process

VLDL secretion

in male and female GPAT1-/- mice after high-sucrose diet
Comment In male and female wild-type mice, the high-fat diet increased myocardial TAG content 1,5 - to 2,2-fold above baseline, respectively, in GPAT1-/- mice, however, heart TAG increased only 35% in males and 80% in females.
Formal Description
Interaction-ID: 2782

environment

high-fat diet

increases_quantity of

drug/chemical compound

Triacylglycerol

in cardiac muscle
Comment The greater myocardial TAG content in female GPAT1-/- mice as compared to male knockout littermates may be due to the significant rise in plasma insulin after 3 months of high-fat feeding.
Formal Description
Interaction-ID: 2784

gene/protein

GPAM

affects_quantity of

complex/PPI

Insulin

in blood; in female GPAT1-/- mice after high-fat diet
Comment Because GPAT1 prefers 16:0-CoA to other long-chain acyl-CoA substrates, the fatty acid composition of heart phospholipids was determined. Compared to wildtype littermate controls, hearts from Gpat1(-/-) mice contained a lower amount of 16:0 in phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine/phosphatidylinositol and significantly more C20:4n6. Phosphatidylcholine and phosphatidylethanolamine from Gpat1(-/-) hearts also contained higher amounts of 18:0 and 18:1.
Formal Description
Interaction-ID: 2787

gene/protein

GPAM

affects_activity of

in cardiac muscle
Comment Although at least three other GPAT isoforms are expressed in the heart, the data suggest that GPAT1 contributes significantly to cardiomyocyte TAG synthesis during lipogenic or high-fat diets and influences the incorporation of 20:4n6 into heart phospholipids.
Formal Description
Interaction-ID: 2800

gene/protein

GPAM

increases_activity of

in cardiac muscle; after lipogenic diet, after high-fat diet
Comment In sucrose-fed male GPAT1-/- mice, plasma TAG and cholesterol were significantly lower than in wild-type mice.
Formal Description
Interaction-ID: 12825

gene/protein

GPAM

affects_quantity of

drug/chemical compound

Cholesterol

in blood; in male GPAT1-/- mice after high-sucrose diet
Drugbank entries Show/Hide entries for
Comment In sucrose-fed female GPAT1-/- mice, values for plasma free fatty acid, TAG, and cholesterol did not differ significantly from control or from treated wild-type mice.
Formal Description
Interaction-ID: 12826

gene/protein

GPAM

NOT affects_quantity of

drug/chemical compound

Triacylglycerol

in blood; in female GPAT1-/- mice after high-sucrose diet
Comment In sucrose-fed female GPAT1-/- mice, values for plasma free fatty acid, TAG, and cholesterol did not differ significantly from control or from treated wild-type mice.
Formal Description
Interaction-ID: 12827

gene/protein

GPAM

NOT affects_quantity of

drug/chemical compound

Cholesterol

in blood; in female GPAT1-/- mice after high-sucrose diet
Drugbank entries Show/Hide entries for