General Information:

Id: 414
Diseases: Diabetes mellitus, type II - [OMIM]
Insulin resistance
Rattus norvegicus
male
primary cells (from male rats) overexpressing mtGPAT1
BTO:0000575 hepatocyte
article
Reference: Lewin TM et al.(2005) Mitochondrial glycerol-3-phosphate acyltransferase-1 directs the metabolic fate of exogenous fatty acids in hepatocytes Am. J. Physiol. Endocrinol. Metab. 288: E835-E844 [PMID: 15598672]

Interaction Information:

Comment Both TAG mass and the incorporation of 14C-oleate into TAG increased in hepatocytes overexpressing mtGPAT1 compared with vector controls.
Formal Description
Interaction-ID: 1812

gene/protein

GPAM

increases_quantity of

drug/chemical compound

Triacylglycerol

if GPAM is overexpressed in primary hepatocytes
Comment Overexpression of mtGPAT1 decreases fatty acid oxidation.
Formal Description
Interaction-ID: 1823

gene/protein

GPAM

decreases_activity of

if GPAM is overexpressed in primary hepatocytes
Comment Despite the marked increases in TAG synthesis and storage in the cells overexpressing mtGPAT1, these cells secreted the same amount of VLDL-TAG as the vector control cells. Thus, in isolated hepatocytes, mtGPAT1 may synthesize a cytosolic pool of TAG that cannot be secreted.
Formal Description
Interaction-ID: 1826

gene/protein

GPAM

increases_activity of

if GPAM is overexpressed in primary hepatocytes
Comment Despite the marked increases in TAG synthesis and storage in the cells overexpressing mtGPAT1, these cells secreted the same amount of VLDL-TAG as the vector control cells. Thus, in isolated hepatocytes, mtGPAT1 may synthesize a cytosolic pool of TAG that cannot be secreted.
Formal Description
Interaction-ID: 12712

gene/protein

GPAM

NOT affects_activity of

process

VLDL triacylglycerol secretion

if GPAM is overexpressed in primary hepatocytes