General Information:

Id: 413
Diseases: Parkinson disease
Homo sapiens
BTO:0000298 COS-7 cell; BTO:0002181 HEK-293T cell; BTO:0000793 SH-SY5Y cell
article
Reference: Greggio E et al.(2006) Kinase activity is required for the toxic effects of mutant LRRK2/dardarin. Neurobiol. Dis. 23: 329-341 [PMID: 16750377]

Interaction Information:

Comment Wildtype LRRK2 transfected into cells was predominantly in the cytoplasm.
Formal Description
Interaction-ID: 1805

gene/protein

LRRK2

is localized in

cellular component

cytoplasm

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1806

gene/protein

LRRK2

NOT increases_quantity of

cellular component

inclusion body

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1807

gene/protein mutant

LRRK2-p.I1122V

increases_quantity of

cellular component

inclusion body

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1808

gene/protein mutant

LRRK2-p.R1441C

increases_quantity of

cellular component

inclusion body

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1809

gene/protein mutant

LRRK2-p.Y1699C

increases_quantity of

cellular component

inclusion body

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1810

gene/protein mutant

LRRK2-p.G2019S

increases_quantity of

cellular component

inclusion body

Comment Wildtype LRRK2 forms only very small inclusion bodies in approximately 5% of cells. In contrast LRRK2 mutants I1122V, R1441C, Y1699C, G2019S and I2020T form larger inclusion bodies in a majority of cells.
Formal Description
Interaction-ID: 1811

gene/protein mutant

LRRK2-p.I2020T

increases_quantity of

cellular component

inclusion body

Comment PD related mutants Y1699C and R1441C of LRRK2 induce nuclear shrinkage 3-4 days after transfection. However the kinase-dead versions of the same mutants produced delayed cell death compared to the kinase-active forms.
Formal Description
Interaction-ID: 1817

gene/protein mutant

LRRK2-p.Y1699C

increases_activity of

process

neuron death

Comment PD related mutants Y1699C and R1441C of LRRK2 induce nuclear shrinkage 3-4 days after transfection. However the kinase-dead versions of the same mutants produced delayed cell death compared to the kinase-active forms.
Formal Description
Interaction-ID: 1818

gene/protein mutant

LRRK2-p.R1441C

increases_activity of

process

neuron death