General Information:

Id: 4,068
Diseases: Alzheimer disease - [OMIM]
Mus musculus
C57BL/6 mouse
BTO:0003037 HT-22 cell
article
Reference: Xie L et al.(2013) Methylene blue induces macroautophagy through 5 adenosine monophosphate-activated protein kinase pathway to protect neurons from serum deprivation Front Cell Neurosci 7: 56 [PMID: 23653592]

Interaction Information:

Comment Methylene blue could protect HT22 hippocampal cell death induced by serum deprivation, companied by induction of macroautophagy. Macroautophagy was induced in the cortex and hippocampus of mouse brains treated with methylene blue.
Formal Description
Interaction-ID: 41904

drug/chemical compound

Methylene blue

increases_activity of

process

macroautophagy

in the cortex and hippocampus
Comment Methylene blue-mediated neuroprotection was abolished by macroautophagy inhibition.
Formal Description
Interaction-ID: 41906

process

macroautophagy

affects_activity of

process

neuroprotection

(methylene blue-mediated neuroprotection)
Comment 5' adenosine-monophosphate-activated protein kinase (AMPK) signaling was activated at 12 and 24h after methylene blue treatment in a dose-dependent manner.
Formal Description
Interaction-ID: 41908

drug/chemical compound

Methylene blue

affects_activity of

complex/PPI

AMPK

Comment Chloroquine is a potent macroautophagy inhibitor.
Formal Description
Interaction-ID: 41910

drug/chemical compound

Chloroquine

decreases_activity of

process

macroautophagy

Drugbank entries Show/Hide entries for Chloroquine
Comment Methylene blue induces macroautophagy via, at partially, AMPK signaling. Both AMPKalpha and AMPKbeta1/2 protein levels were increased in vitro.
Formal Description
Interaction-ID: 41912

drug/chemical compound

Methylene blue

increases_expression of

gene/protein

PRKAA1

in vitro
Drugbank entries Show/Hide entries for PRKAA1
Comment Methylene blue act as an alternative electron carrier in the mitochondrial electron transport chain to enhance ATP production while energy is in short.
Formal Description
Interaction-ID: 41915

drug/chemical compound

Methylene blue

increases_activity of

while energy is in short
Comment Methylene blue induces macroautophagy via, at partially, AMPK signaling. Both AMPKalpha and AMPKbeta1/2 protein levels were increased in vitro.
Formal Description
Interaction-ID: 41916

drug/chemical compound

Methylene blue

increases_expression of

gene/protein

PRKAA2

in vitro
Comment Methylene blue induces macroautophagy via, at partially, AMPK signaling. Both AMPKalpha and AMPKbeta1/2 protein levels were increased in vitro.
Formal Description
Interaction-ID: 41917

drug/chemical compound

Methylene blue

increases_expression of

gene/protein

PRKAB1

in vitro
Drugbank entries Show/Hide entries for PRKAB1
Comment Methylene blue induces macroautophagy via, at partially, AMPK signaling. Both AMPKalpha and AMPKbeta1/2 protein levels were increased in vitro.
Formal Description
Interaction-ID: 41919

drug/chemical compound

Methylene blue

increases_expression of

gene/protein

PRKAB2

in vitro
Drugbank entries Show/Hide entries for PRKAB2
Comment Methylene blue-induced AMPK activation was accompanied by higher levels of LC3B type II, the activated form of LC3 participating autophagosome formation.
Formal Description
Interaction-ID: 41929

complex/PPI

AMPK

increases_quantity of

gene/protein

MAP1LC3B

if AMPK activation is Methylene blue-induced