General Information:

Id: 385
Diseases: Amyotrophic lateral sclerosis
Homo sapiens
BTO:0003350 BV-2 cell
article
Reference: Liu Y et al.(2009) Expression of amyotrophic lateral sclerosis-linked SOD1 mutant increases the neurotoxic potential of microglia via TLR2 J. Biol. Chem. 284: 3691-3699 [PMID: 19091752]

Interaction Information:

Comment Mutant SOD1 (G93A or L8Q or G10V) enhances TLR2 stimulation-induced microglial TNF-alpha secretion. Mutant SOD1 (G93A or L8Q or G10V)-overexpressing cells released significantly more TNF-alpha than wtSOD1-overexpressing or mock control cells after TLR2 stimulation. Secretion of IL-6 was reduced.
Formal Description
Interaction-ID: 1578

gene/protein mutant

SOD1-p.G93A

increases_quantity of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for TNF
Comment Mutant SOD1 (G93A or L8Q or G10V) enhances TLR2 stimulation-induced microglial TNF-alpha secretion. Mutant SOD1 (G93A or L8Q or G10V)-overexpressing cells released significantly more TNF-alpha than wtSOD1-overexpressing or mock control cells after TLR2 stimulation. Secretion of IL-6 was reduced.
Formal Description
Interaction-ID: 1582

gene/protein mutant

SOD1-p.G93A

decreases_quantity of

gene/protein

IL6

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for IL6
Comment SOD1 G93A mutation does not enhance transcription of TNF-alpha.
Formal Description
Interaction-ID: 1583

gene/protein mutant

SOD1-p.G93A

NOT increases_expression of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for TNF
Comment When oxidative stress upon TLR2 or TLR4 stimulation was compared between different cell types, mSOD1(G93A) overexpression was observed to result in significantly higher levels of H2O2 and lower concentrations of thiol groups in the cell lysate.
Formal Description
Interaction-ID: 1586

gene/protein mutant

SOD1-p.G93A

increases_quantity of

drug/chemical compound

H2O2

in TLR2 or TLR4 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Comment When oxidative stress upon TLR2 or TLR4 stimulation was compared between different cell types, mSOD1(G93A) overexpression was observed to result in significantly higher levels of H2O2 and lower concentrations of thiol groups in the cell lysate.
Formal Description
Interaction-ID: 1592

gene/protein mutant

SOD1-p.G93A

increases_activity of

in TLR2 or TLR4 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Comment P47-PHOX mediates SOD1 G93A mutation-enhanced oxidative stress and subsequent TNF-alpha secretion following TLR2 stimulation.
Formal Description
Interaction-ID: 1595

gene/protein

NCF1

affects_activity of

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Comment P47-PHOX mediates SOD1 G93A mutation-enhanced oxidative stress and subsequent TNF-alpha secretion following TLR2 stimulation.
Formal Description
Interaction-ID: 1598

gene/protein

NCF1

affects_quantity of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for TNF
Comment Pretreatment of microglial cells with antioxidant regents, e.g. NAC (N-acetyl-L-cysteine) and catalase, before TLR2 stimulation, showed that SOD1 G93A overexpression-associated enhancement of TNF-alpha secretion was reversed.
Formal Description
Interaction-ID: 1599

drug/chemical compound

Acetylcysteine

decreases_quantity of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for Acetylcysteine or TNF
Comment ADAM10 and 17 are involved in SOD1 G93A mutation-enhanced TNF-alpha secretion following TLR2 stimulation. Following ADAM10 or ADAM17 gene silencing, the mSOD1(G93A)-induced enhancement of microglial TNF-alpha release was reversed.
Formal Description
Interaction-ID: 1605

gene/protein

ADAM10

affects_quantity of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for TNF
Comment ADAM10 and 17 are involved in SOD1 G93A mutation-enhanced TNF-alpha secretion following TLR2 stimulation. Following ADAM10 or ADAM17 gene silencing, the mSOD1(G93A)-induced enhancement of microglial TNF-alpha release was reversed.
Formal Description
Interaction-ID: 1606

gene/protein

ADAM17

affects_quantity of

gene/protein

TNF

in TLR2 stimulated murine microglial cell line BV-2; if SOD1(G93A) is overexpressed
Drugbank entries Show/Hide entries for ADAM17 or TNF
Comment Mutant SOD1 (G93A or L8Q or G10V) enhances TLR2 stimulation-induced microglial TNF-alpha secretion. Mutant SOD1 (G93A or L8Q or G10V)-overexpressing cells released significantly more TNF-alpha than wtSOD1-overexpressing or mock control cells after TLR2 stimulation. Secretion of IL-6 was reduced. The secretion of IL-1-beta was not significantly increased by the TLR2 stimulation.
Formal Description
Interaction-ID: 1607

gene/protein mutant

SOD1-p.G93A

NOT affects_quantity of

gene/protein

IL1B

in TLR2 stimulated murine microglial cell line BV-2; if mutSOD1 is overexpressed
Drugbank entries Show/Hide entries for IL1B