General Information:

Id: 377
Diseases: Amyotrophic lateral sclerosis
Mus musculus
SOD1(G93A) transgenic mouse
BTO:0001279 spinal cord
article
Reference: Tortarolo M et al.(2003) Persistent activation of p38 mitogen-activated protein kinase in a mouse model of familial amyotrophic lateral sclerosis correlates with disease progression Mol. Cell. Neurosci. 23: 180-192 [PMID: 12812752]

Interaction Information:

Comment Accumulation of p38MAPK was found by immunoblotting in the spinal cord of G93A mice during the progression of disease, but no changes were detected in its mRNA levels.
Formal Description
Interaction-ID: 1534

gene/protein mutant

SOD1-p.G93A

increases_quantity of

gene/protein

p38 MAPK

in spinal cord
Comment In SOD1G93A mice with advanced disease (19 weeks), p38MAPK-P fluorescence was increased in the whole gray matter of the spinal cord including the substantia gelatinosa.
Formal Description
Interaction-ID: 1536

gene/protein mutant

SOD1-p.G93A

increases_activity of

gene/protein

p38 MAPK

in spinal cord
Comment The experiments showed colocalization of p38MAPK-P and neurofilaments. In SOD1G93A mice, at all ages examined, there is intense immunofluorescence of SMI31 (antibody for phosphorylated neurofilament H and M) in the vacuolized motor neurone cell bodies and colocalization of the two antibodies (p38MAPK-P and SMI31) in these cells.
Formal Description
Interaction-ID: 1537

gene/protein

p38 MAPK

interacts (colocalizes) with

protein modification

NEFH-mod

Comment The experiments showed colocalization of p38MAPK-P and neurofilaments. In SOD1G93A mice, at all ages examined, there is intense immunofluorescence of SMI31 (antibody for phosphorylated neurofilament H and M) in the vacuolized motor neurone cell bodies and colocalization of the two antibodies (p38MAPK-P and SMI31) in these cells.
Formal Description
Interaction-ID: 1538

gene/protein

p38 MAPK

interacts (colocalizes) with

protein modification

NEFM-mod

Comment The experiments showed colocalization of p38MAPK-P and glial fibrillary acidic protein (GFAP). In transgenic SOD1G93A mice at the presymptomatic stage (8 weeks) no colocalization was found with p38MAPK-P, at the late stage of disease (19 weeks) astrocytes appeared strongly labeled with both GFAP and p38MAPK-P antibodies.
Formal Description
Interaction-ID: 1539

gene/protein

p38 MAPK

interacts (colocalizes) with

gene/protein

GFAP

in astrocytes; at the age of 19 weeks
Comment The experiments showed colocalization of p38MAPK-P and CD11b in the ventral horn spinal cord of 19-week-old SOD1G93A mice.
Formal Description
Interaction-ID: 1540

gene/protein

p38 MAPK

interacts (colocalizes) with

gene/protein

ITGAM

in spinal cord; at the age of 19 weeks
Comment Accumulation of p38MAPK was found by immunoblotting in the spinal cord of G93A mice during the progression of disease, but no changes were detected in its mRNA levels.
Formal Description
Interaction-ID: 13499

gene/protein mutant

SOD1-p.G93A

NOT increases_expression of

gene/protein

p38 MAPK

in spinal cord