General Information:

Id: 3,040
Diseases: Diabetes mellitus, type II - [OMIM]
Insulin resistance
Major depressive disorder - [OMIM]
Mus musculus
male
article
Reference: Lambillotte C et al.(1997) Direct glucocorticoid inhibition of insulin secretion. An in vitro study of dexamethasone effects in mouse islets. J. Clin. Invest. 99: 414-423 [PMID: 9022074]

Interaction Information:

Comment The direct effects of glucocorticoids on pancreatic beta cell function were studied with normal mouse islets. Dexamethasone inhibited insulin secretion from cultured islets in a concentration-dependent manner: maximum of approximately 75% at 250 nM and IC50 at approximately 20 nM dexamethasone. This inhibition was of slow onset and only slowly reversible. It was prevented by a blocker of nuclear glucocorticoid receptors, by pertussis toxin, by a phorbol ester, and by dibutyryl cAMP, but was unaffected by an increase in the fuel content of the culture medium. Dexamethasone also inhibited insulin secretion induced by arginine, tolbutamide, or high K+.
Formal Description
Interaction-ID: 27966

drug/chemical compound

Dexamethasone

decreases_activity of

in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone
Comment Dexamethasone treatment did not affect islet cAMP levels but slightly reduced inositol phosphate formation.
Formal Description
Interaction-ID: 27967

drug/chemical compound

Dexamethasone

NOT affects_quantity of

drug/chemical compound

cAMP

in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone or cAMP
Comment The inhibition of the insulin secretion by dexamethasone could not be ascribed to a lowering of insulin stores (higher in dexamethasone-treated islets), to an alteration of glucose metabolism (glucose oxidation and NAD(P)H changes were unaffected), or to a lesser rise of cytoplasmic Ca2+ in beta cells (only the frequency of the oscillations was modified).
Formal Description
Interaction-ID: 27968

drug/chemical compound

Dexamethasone

increases_activity of

process

insulin storage

in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone
Comment The inhibition of the insulin secretion by dexamethasone could not be ascribed to a lowering of insulin stores (higher in dexamethasone-treated islets), to an alteration of glucose metabolism (glucose oxidation and NAD(P)H changes were unaffected), or to a lesser rise of cytoplasmic Ca2+ in beta cells (only the frequency of the oscillations was modified).
Formal Description
Interaction-ID: 27969

drug/chemical compound

Dexamethasone

NOT affects_activity of

in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone
Comment The inhibition of the insulin secretion by dexamethasone could not be ascribed to a lowering of insulin stores (higher in dexamethasone-treated islets), to an alteration of glucose metabolism (glucose oxidation and NAD(P)H changes were unaffected), or to a lesser rise of cytoplasmic Ca2+ in beta cells (only the frequency of the oscillations was modified).
Formal Description
Interaction-ID: 27970

drug/chemical compound

Dexamethasone

NOT affects_quantity of

drug/chemical compound

Ca2+

in cytoplasm, in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone
Comment This inhibition of the insulin secretion by dexamethasone is brought about by a decrease in the effectiveness of cytoplasmic Ca21 on the secretory process.
Formal Description
Interaction-ID: 27971

drug/chemical compound

Dexamethasone

decreases_activity of

drug/chemical compound

Ca2+

in cytoplasm, in pancreas, in pancreatic beta cells
Drugbank entries Show/Hide entries for Dexamethasone
Comment This inhibition of the insulin secretion by dexamethasone is brought about by a decrease in the effectiveness of cytoplasmic Ca21 on the secretory process.
Formal Description
Interaction-ID: 27972

drug/chemical compound

Ca2+

increases_activity of

in cytoplasm, in pancreas, in pancreatic beta cells