General Information:

Id: 2,186
Diseases: Diabetes mellitus, type II - [OMIM]
Insulin resistance
Obesity - [OMIM]
Mus musculus
BTO:0000443 adipocyte
article
Reference: Talior I et al.(2003) Increased glucose uptake promotes oxidative stress and PKC-delta activation in adipocytes of obese, insulin-resistant mice. Am. J. Physiol. Endocrinol. Metab. 285: E295-E302 [PMID: 12857675]

Interaction Information:

Comment The adipocytes used were isolated either from C57Bl/6J mice that were raised on a high-fat diet (HF) and developed obesity and insulin resistance or from control animals. Basal glucose uptake significantly increased (8-fold) in HF adipocytes, and this was accompanied with upregulation of GLUT1 expression levels.
Formal Description
Interaction-ID: 18305

environment

high-fat diet

increases_expression of

gene/protein

SLC2A1

in adipose tissue
Comment Insulin-induced glucose uptake was inhibited in HF adipocytes and GLUT4 content reduced by 20% in these adipocytes.
Formal Description
Interaction-ID: 18306

environment

high-fat diet

decreases_expression of

gene/protein

SLC2A4

in adipose tissue
Comment Insulin-induced glucose uptake was inhibited in HF adipocytes and GLUT4 content reduced by 20% in these adipocytes.
Formal Description
Interaction-ID: 18307

environment

high-fat diet

decreases_activity of

process

glucose import

in adipose tissue; if induced by insulin and mediated by GLUT4
Comment The adipocytes used were isolated either from C57Bl/6J mice that were raised on a high-fat diet (HF) and developed obesity and insulin resistance or from control animals. Basal glucose uptake significantly increased (8-fold) in HF adipocytes, and this was accompanied with upregulation of GLUT1 expression levels.
Formal Description
Interaction-ID: 18308

environment

high-fat diet

increases_activity of

process

glucose import

in adipose tissue; if mediated by GLUT1
Comment Reactive oxygen species (ROS) increased twofold in HF adipocytes compared with control adipocytes and were largely reduced with decreased glucose concentrations. At zero glucose, ROS levels were reduced to the normal levels seen in control adipocytes.
Formal Description
Interaction-ID: 18309

environment

high-fat diet

increases_quantity of

drug/chemical compound

Reactive oxygen species

in adipose tissue
Comment The activity of PKC-delta increased twofold in HF adipocytes compared with control adipocytes and was further activated by H2O2. Moreover, PKC-delta activity was inhibited in HF adipocytes either by glucose deprivation or by treatment with the antioxidant N-acetyl-l-cysteine.
Formal Description
Interaction-ID: 18311

environment

high-fat diet

increases_activity of

gene/protein

PRKCD

in adipose tissue
Drugbank entries Show/Hide entries for PRKCD
Comment The activity of PKC-delta increased twofold in HF adipocytes compared with control adipocytes and was further activated by H2O2. Moreover, PKC-delta activity was inhibited in HF adipocytes either by glucose deprivation or by treatment with the antioxidant N-acetyl-l-cysteine.
Formal Description
Interaction-ID: 18316

drug/chemical compound

H2O2

increases_activity of

gene/protein

PRKCD

in adipose tissue
Drugbank entries Show/Hide entries for PRKCD
Comment Increased glucose intake in HF adipocytes increases oxidative stress, which in turn promotes the activation of PKC-delta.
Formal Description
Interaction-ID: 18317

process

glucose import

increases_activity of

in adipose tissue
Comment Increased glucose intake in HF adipocytes increases oxidative stress, which in turn promotes the activation of PKC-delta.
Formal Description
Interaction-ID: 18318

increases_activity of

gene/protein

PRKCD

in adipose tissue
Drugbank entries Show/Hide entries for PRKCD