General Information:

Id: 1,538 (click here to show other Interactions for entry)
Diseases: Parkinson disease
Mammalia
BTO:0004476 MES-23.5 cell
article
Reference: Xu H et al.(2010) Rg1 protects iron-induced neurotoxicity through antioxidant and iron regulatory proteins in 6-OHDA-treated MES23.5 cells J. Cell. Biochem. 111: 1537-1545 [PMID: 20872781]

Interaction Information:

Comment Iron treatment decreased the mitochondrial membrane potential. Rg1 pretreatment could restore the mitochondrial membrane potential to the control. This indicated Rg1 could protect iron-induced damage of dopamine neurons.
Formal Description
Interaction-ID: 10975

drug/chemical compound

Iron

increases_activity of

by decreasing the mitochondrial membrane potential
Comment Iron treatment decreased the mitochondrial membrane potential. Rg1 pretreatment could restore the mitochondrial membrane potential to the control. This indicated Rg1 could protect iron-induced damage of dopamine neurons.
Formal Description
Interaction-ID: 10977

drug/chemical compound

Iron

increases_activity of

process

neuron death

by decreasing the mitochondrial membrane potential
Comment Iron treatment decreased the mitochondrial membrane potential. Rg1 pretreatment could restore the mitochondrial membrane potential to the control. This indicated Rg1 could protect iron-induced damage of dopamine neurons.
Formal Description
Interaction-ID: 10978

drug/chemical compound

Ginsenoside Rg1

decreases_activity of

process

neuron death

if neuron death has been induced by iron
Comment Iron treatment decreased the mitochondrial membrane potential. Rg1 pretreatment could restore the mitochondrial membrane potential to the control. This indicated Rg1 could protect could protect iron-induced damage of dopamine neurons.
Formal Description
Interaction-ID: 10979

drug/chemical compound

Ginsenoside Rg1

decreases_activity of

drug/chemical compound

Iron

by decreasing iron-induced mitochondrial membrane depolarisation
Comment 6-OHDA induces an increase in iron influx.
Formal Description
Interaction-ID: 10980

drug/chemical compound

6-OHDA

increases_quantity of

drug/chemical compound

Iron

by increasing iron influx
Comment Rg1 treatment prevented ferrous iron influx in 6-OHDA treated MES23.5 cells.
Formal Description
Interaction-ID: 10981

drug/chemical compound

Ginsenoside Rg1

decreases_quantity of

drug/chemical compound

Iron

by decreasing 6-OHDA-induced iron influx
Comment There was a 2.41-fold increase of DMT1+IRE mRNA in 6-OHDA treated cells. Pretreatment with Rg1 reversed the increase of DMT1+IRE mRNA.
Formal Description
Interaction-ID: 10982

drug/chemical compound

6-OHDA

increases_expression of

gene/protein

SLC11A2

Comment There was a 2.41-fold increase of DMT1+IRE mRNA in 6-OHDA treated cells. Pretreatment with Rg1 reversed the increase of DMT1+IRE mRNA.
Formal Description
Interaction-ID: 10983

drug/chemical compound

Ginsenoside Rg1

decreases_expression of

gene/protein

SLC11A2

if expression has been induced by 6-OHDA
Comment DMT1 is a ferrous iron importer protein.
Formal Description
Interaction-ID: 10984

gene/protein

SLC11A2

increases_quantity of

drug/chemical compound

Iron

by increasing the import of iron
Comment DMT1 is a ferrous iron importer protein.
Formal Description
Interaction-ID: 10985

gene/protein

SLC11A2

increases_transport of

drug/chemical compound

Iron

by increasing the import of iron
Comment Both IRP1 and IRP2 were up-regulated in mRNA and protein levels after 6-OHDA treatment compared to the control.
Formal Description
Interaction-ID: 10986

drug/chemical compound

6-OHDA

increases_expression of

gene/protein

ACO1

Comment Both IRP1 and IRP2 were up-regulated in mRNA and protein levels after 6-OHDA treatment compared to the control.
Formal Description
Interaction-ID: 10987

drug/chemical compound

6-OHDA

increases_expression of

gene/protein

IREB2

Comment After Rg1 pretreatment the mRNA and protein levels of IRPs restored to the levels of control.
Formal Description
Interaction-ID: 10988

drug/chemical compound

Ginsenoside Rg1

decreases_quantity of

gene/protein

ACO1

Comment After Rg1 pretreatment the mRNA and protein levels of IRPs restored to the levels of control.
Formal Description
Interaction-ID: 10989

drug/chemical compound

Ginsenoside Rg1

decreases_quantity of

gene/protein

IREB2

Comment Both IRP1 and IRP2 were up-regulated in mRNA and protein levels after 6-OHDA treatment compared to the control.
Formal Description
Interaction-ID: 10990

drug/chemical compound

6-OHDA

increases_quantity of

gene/protein

ACO1

Comment Both IRP1 and IRP2 were up-regulated in mRNA and protein levels after 6-OHDA treatment compared to the control.
Formal Description
Interaction-ID: 10991

drug/chemical compound

6-OHDA

increases_quantity of

gene/protein

IREB2

Comment IRPs are RNA-binding proteins that affect the translation and stabilization of specific mRNAs, like DMT1, by binding to IREs in iron related proteins and maintain the iron levels.
Formal Description
Interaction-ID: 10992

gene/protein

ACO1

affects_activity of

gene/protein

SLC11A2

Comment IRPs are RNA-binding proteins that affect the translation and stabilization of specific mRNAs, like DMT1, by binding to IREs in iron related proteins and maintain the iron levels.
Formal Description
Interaction-ID: 10993

gene/protein

IREB2

affects_activity of

gene/protein

SLC11A2

Comment Rg1 treatment prevented ferrous iron influx in 6-OHDA treated MES23.5 cells.
Formal Description
Interaction-ID: 11109

drug/chemical compound

Ginsenoside Rg1

affects_activity of

by decreasing 6-OHDA-induced iron influx