General Information:

Id: 116
Diseases: Alzheimer disease - [OMIM]
Homo sapiens
BTO:0000007 HEK-293 cell
article
Reference: Alessi DR et al.(1996) Mechanism of activation of protein kinase B by insulin and IGF-1 EMBO J. 15: 6541-6551 [PMID: 8978681]

Interaction Information:

Comment After transfection into 293 cells AKT1 was activated 20- and 50-fold in response to insulin and IGF-1 respectively.
Formal Description
Interaction-ID: 389

complex/PPI

Insulin

increases_activity of

gene/protein

AKT1

Drugbank entries Show/Hide entries for AKT1
Comment After transfection into 293 cells AKT1 was activated 20- and 50-fold in response to insulin and IGF-1 respectively.
Formal Description
Interaction-ID: 391

gene/protein

IGF1

increases_activity of

gene/protein

AKT1

Drugbank entries Show/Hide entries for IGF1 or AKT1
Comment The activation of AKT1 was accompanied by its phosphorylation at Thr308 and Ser473 and, like activation, phosphorylation of both of these residues was prevented by the phosphatidylinositol 3-kinase inhibitor wortmannin.
Formal Description
Interaction-ID: 393

complex/PPI

Insulin

affects_phosphorylation of

gene/protein

AKT1

at Thr308 and Ser473
Drugbank entries Show/Hide entries for AKT1
Comment The activation of AKT1 was accompanied by its phosphorylation at Thr308 and Ser473 and, like activation, phosphorylation of both of these residues was prevented by the phosphatidylinositol 3-kinase inhibitor wortmannin.
Formal Description
Interaction-ID: 394

gene/protein

IGF1

affects_phosphorylation of

gene/protein

AKT1

at Thr308 and Ser473
Drugbank entries Show/Hide entries for IGF1 or AKT1
Comment Wortmannin is a phosphatidylinositol 3-kinase inhibitor.
Formal Description
Interaction-ID: 397

drug/chemical compound

Wortmannin

decreases_phosphorylation of

gene/protein

AKT1

at Thr308 and Ser473
Drugbank entries Show/Hide entries for AKT1
Comment MAPKAP kinase-2 phosphorylates Ser473 of AKT1 causing partial activation.
Formal Description
Interaction-ID: 401

gene/protein

MAPKAPK2

increases_phosphorylation of

gene/protein

AKT1

at Ser473
Drugbank entries Show/Hide entries for MAPKAPK2 or AKT1
Comment MAPKAP kinase-2 phosphorylates Ser473 of AKT1 causing partial activation.
Formal Description
Interaction-ID: 404

gene/protein

MAPKAPK2

increases_activity of

gene/protein

AKT1

by phosphorylation at Ser473
Drugbank entries Show/Hide entries for MAPKAPK2 or AKT1
Comment AKT1-Phosphorylation of Thr308 is not dependent on AKT1-phosphorylation of Ser473 or vice versa in 293 cells.
Formal Description
Interaction-ID: 405

protein modification

AKT1-phosThr308

NOT affects_phosphorylation of

protein modification

AKT1-phosSer473

in 293 cells
Comment AKT1-Phosphorylation of Thr308 is not dependent on AKT1-phosphorylation of Ser473 or vice versa in 293 cells.
Formal Description
Interaction-ID: 12845

protein modification

AKT1-phosSer473

NOT affects_phosphorylation of

protein modification

AKT1-phosThr308

in 293 cells
Comment Wortmannin is a phosphatidylinositol 3-kinase inhibitor.
Formal Description
Interaction-ID: 12850

drug/chemical compound

Wortmannin

decreases_activity of

complex/PPI

Phosphatidylinositol 3-kinase

Comment The activation of AKT1 was accompanied by its phosphorylation at Thr308 and Ser473 and, like activation, phosphorylation of both of these residues was prevented by the phosphatidylinositol 3-kinase inhibitor wortmannin.
Formal Description
Interaction-ID: 12851

drug/chemical compound

Wortmannin

decreases_quantity of

protein modification

AKT1-phosSer473

Comment The activation of AKT1 was accompanied by its phosphorylation at Thr308 and Ser473 and, like activation, phosphorylation of both of these residues was prevented by the phosphatidylinositol 3-kinase inhibitor wortmannin.
Formal Description
Interaction-ID: 12852

drug/chemical compound

Wortmannin

decreases_quantity of

protein modification

AKT1-phosThr308